A variety of peptides are becoming more available on the market today. While peptides are not yet approved for human use, research has indicated that these chemicals have a strong potential for managing different conditions through pharmaceutical application in the future. Research today focuses greatly on identifying new peptides, determining if these chemicals match the reactions seen in animal test subjects, determining if these peptides can be harvested or recreated and observing the effects of applying peptides to test subjects.
As research continues these peptides are gaining attention in the public market, which is making it easier for research facilities to get the tools they require.
Peptide research is quickly becoming one of the largest branches of pharmaceutical research worldwide. There are a number of laboratories that focus solely on this type of research, both in conducting their own experiments and preparing tools and research that can be used by other companies to further the study of animal peptides.
Much of the research that is being created by these companies is being shared to ensure that new discoveries are not being repeated, wasting time and resources. Working together also allows research firms to sell the products they have developed to other experimenters, guaranteeing a higher quality product and shortening the time it takes to confirm results.
Large Application Peptides
Peptides that are produced in large batches can be purchases, often at a lower price than purchasing individual applications because of the altering designs used to create these chemicals.
- Peptides that can be produced in large quantities can only be sold to customers if a certain level of purity can be confirmed. This helps to ensure consistency throughout the peptide sample so it can be applied over time and in a variety of application sizes with somewhat predictable results.
- If there are errors in a large batch of peptides it can result in data sets that cannot be used as a benchmark for further study. This can cost research facilities and the peptide research community a great deal of money as they need to repeat experiments to confirm findings when they could otherwise be given more credit.
- The methods for creating large application sizes of peptides have become increasingly refined which has greatly lowered the cost of investing in these peptide sets for research. This, in turn, is increasing the accuracy of data because peptide experiments can be run on larger groups of test subjects as well as in larger amounts of repeat studies over time.
Peptides are very fragile, so if buying large quantities of peptides it is important to take adequate precautions to ensure that they maintain their purity for as long as possible. Peptides are typically designed to be kept at cold temperatures when they are not in use. The specific temperature that is considered ideal for a given peptide should be noted on the container.
Bring any amount of peptide you plan to use up to temperature slowly and wait until it has reached the appropriate temperature before reconstituting an application. Keep any amount of peptide that will not be used immediately frozen as constant thawing and refreezing will damage the structure of the amino acids.
Understanding Purity Testing
It is not uncommon to see peptide companies advertising purity levels on their websites and sale pages, but it is important to understand the methodology that is used to determine these purity standards for best results in peptide research with these products.
- There are different methodologies that are used to create peptide bonds in synthetic forms. These different methods will yield different results when they are completed. Different methodologies are more or less effective depending on the type of peptide that the laboratory is creating and how it is designed to function when it is applied to animal tissues later.
- HPLC is a method that combines a variety of compounds and analyzes how they break down to determine what components were included in this mixture. This methodology is not designed to identify specific compounds or to provide you with the Dalton mass of compounds for cross referencing.
- Those that are already aware of the compounds that went into a mixture can use this methodology as a means of determining the purity of the mixture, though this is less effective if there are multiple compounds in the mixture. This should be noted if a sales site claims that it uses this methodology as a means of testing purity because the end result may not be particularly accurate.
- SDS page refers to sodium gel electrophoresis that is commonly used in chemistry, forensics or genetics as a means of identifying compounds. In this case proteins are separated using electrophoretic mobility that divides the electric charges in the polypeptide chains. This will create a band in a gel sequence that can be used to identify proteins that exist at different weights. This is ideal for identifying proteins or peptides that are well known because a specific weight distribution can be checked to determine if these peptides are present. While SDS page is essential for determining if specific peptides are present it cannot be used to determine how much of a compound is present in a given sample.
- Mass spectrometry can be used when single spectrum masses of molecules need to be displayed for an individual sample. This is considered to be the most accurate way of measuring the purity of a sample of peptides used for research. This method determines the elemental composition of a peptide sample by checking the molecule mass weight. If researchers are aware of the goal weight their sample should be, this can be used to see how accurate the given sample is, which is essential when creating variants of a given peptide.
The only true way to determine accuracy beyond doubt is to perform a full amino acid analysis because this will show the percentage of amino acids present and whether or not this matches up with what a sample should be. This is not considered feasible for bulk productions of peptides, though random samplings may be taken for a full amino acid analysis to check that a production method is producing accurate results.
Buying Peptides from a Trustworthy Source
Given the wide variance in quality that can stem from different production methods, it is important to perform careful research when selecting a company to purchase peptides from.
- Maxim avoids making any claims regarding the purity of peptide compounds unless they have been tested for these purity levels.
- All peptides have been created with the intention of being used in a research setting, meaning there is a higher requirement for purity and accuracy of the compound.
- No peptides are sold in constituted form. This helps to ensure that they remain stable during shipping and can be stored for a longer length of time before use.
Like any type of research product, all instructions for the use and storage of a peptide should be read carefully both before a final purchase is made and before the peptide is used in a laboratory setting.
Maintaining the proper temperature profile, keeping the peptide in a sterile, sealed container and applying them to animal test subjects in the proper size applications can help to ensure that the peptide behaves properly without jeopardizing the accuracy of any results that stem from these trials. All information necessary to follow these steps should be included with every purchase.
PEAKS Database Search Tutorial on Peptide Identification