Category Archives: Case Studies

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Ostarine is also known as enodosarm when it is sold for clinical study. This chemical is being developed to mimic the effects of an andogen receptor modulator. The hope is to eventually develop this chemical so it can be used to help manage muscle wasting or small cell lung cancer in animals.

This chemical is not yet ready for study or treatment of humans but it has been used in a variety of animal test subjects to determine the effects of this chemical. Ostarine is also currently banned by the World Anti-Doping Agency, given its potential for abuse if it were to be released to the public, though this does not impact its potential for future medical use once this has been better developed.

As it has been developed Ostarine has taken on a variety of different names but the structure is referred to as GTx-024 or MK-2866. This refers specifically to the peptide’s nature as an investigational selective andogen receptor modulator or SARM. The chemical is developed by GTX Inc. but was formerly produced by Merck and Company.

This chemical is commonly confused or linked with andarine which has a different chemical structure and does not produce the same results in clinical studies that Ostarine was designed to manage.

Clinical Trials and Further Study

At this time the manufacturer GTx has used Ostarine in clinical trials that have included around 1200 animal test subjects which have included five efficacy studies.

The company announced recently that they had finished compiling results from two of the phase 3 clinical trials of power 1 and 2 of the Ostarine formula which is used to address muscle wasting and help to prevent this condition in animal test subjects that were subjected to NSCLC.

In each of these studies the results were derived from placebo-controlled and double blind clinical trials which included at least 325 test subjects that either had NSCLC in stage three or four severity. These test subjects were randomly assigned to receive oral applications of enobosarm or placebo chemicals at 3mg daily. At this time they started the doublet chemotherapy (standard platinum) as well.

During these power trials the goal was to assess the response of the Ostarine, compared to the placebo chemicals in order to determine the co-primary endpoints at three month intervals of the application and chemotherapy process. At this time the total lean body mass of the animal was tested which was then assessed by DXA and the physical function by encouraging minor exercise in the animal.

• The durability of applications of Ostarine was also assessed at the 5 month point in the study to better understand the long term effects these treatments would have on the muscle tissue and the overall power that the animal could manage.

When these results were published, GTx announced that the results of this study had failed in meeting the set co-primary endpoints that had been set for lean body mass as well as the physical function of the animal. These were statistically assessed using responder analysis but data from the study of the Ostarine applications showed a consistent effect that could improve or maintain the lean body mass compared to a placebo chemical application.

• This could be associated with the potential longevity with animals that managed to survive their condition for longer periods of time, regardless of the application of chemicals or other existing treatment plans that they were subjected to.
• Generally, Ostarine was found to be well tolerated with only few serious adverse reactions occurring in groups receiving the chemical application or the placebo, which has caused the FDA to begin considering this chemical as a potential future treatment possibility for muscle wasting disease.

The chemical formula that is used to create Ostarine has not been disclosed to the public but the chemical composition of the formula has been listed in patent databases, including the WIPO. Primary literature at Zhang also mentioned this formula in 2009. There are a variety of SARM chemotypes on the market for research purposes today but aryl propionamides including andarine/S-4 or Ostarine are some of the more advanced chemicals that are being investigated for their potential aproperties.

The atom connectivity of Ostarine is different from andarine because the cyano substitutions of the phenyl rings relace the acetamido and nitro moieties.

Resource Box:

http://en.wikipedia.org/wiki/Enobosarm

http://www.gtxinc.com/Pipeline/OstarineMK2866.aspx?Sid=4

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The structure and chemical integrity of a peptide plays a strong role in the way that this peptide interacts with biological components. Peptides may be designed to interact with specific cells or tissues, and are therefore given the opportunity to bond or break—based on the needs of these areas. The way a peptide breaks or continues to bond with structures throughout the body will determine the biological process that is completed as well as the success of this function once it has begun.

The ability to create peptides with sound bonds is essential both to natural peptides formed by the body and synthetic peptides that are applied to animals for research purposes. The sooner a chemical bond within a peptide is broken, the shorter the effective life of this peptide will be.

If a peptide does not have an effective structure, it may be vulnerable to damage on the shelf, which can alter research data or create unexpected side effects in animal test subjects upon the  chemicals being applied. There are a variety of products and chemical methods that are used to address this growing need to ensure that peptides that could one day be used for pharmacological purposes will behave in a consistent manner.

3.5.5 Discuss the relationship between one gene and one polypeptide:

IPam

Ipam is an abbreviation for NNC 26-0161 more commonly known as ipamorelin, a polypeptide hormone that acts as a secretagogue and mimic to ghrelin that was developed by Novo Nordisk.

• This synthetic peptide was developed in the 1990s as part of a group of peptides designed to mimic GHRPs that are developed within the bodies of animals. Ipam is capable of encouraging an animal’s body to release a large amount of growth hormone without the need for additional peptides to be present. This is because the peptide suppresses somatostatin which normally acts as an antagonist to GHRH and stimulates the anterior pituitary to release growth hormone the way GHRP-6 or GHRP-2 would.

• Similar to GHRP-2, Ipam does not affect the lipogenic properties of ghrelin, but unlike GHRP-6 Ipam does not induce hunger in the animals when it is applied.

• Ipam can act synergistically if it is applied while natural GHRH is present in an animal’s system or when it is administered alongside a GHRH analog or GHRH itself. Common analogs include GRF 1-29 or semorelin.

• The synergy of these peptides stems from the somatostatin suppression and the increase in growth hormone per somatotrope. GHRH increases somatotropes that increase growth hormone but Ipam creases a neuronal excitation of the animal’s hypothalamus that can last up to 3 hours after the chemical is applied. This is a similar reaction to GHRP-2 or GHRP-6.

• Compared to other chemicals in the GHRP family, Ipam has a unique set of properties known as selectiveness. In one study growth hormone secretagogue acted with an increasing efficiency and potency in vivo and in vitro. This led to the chemical being identified as one of several compounds that lacks the central dipeptide ala-trp that is commonly seen in GHRP-1.

• Ipamorelin caused growth hormone releases in the pituitary gland of rats in vitro—with a similar potency of GHRP-6—which allowed researchers to profile this chemical as a growth hormone releasing hormone with an antagonist structure that was capable of stimulating growth hormone via the GHRP receptors.

• Research in pentobarbital rats that were anaesthetized, Ipam was capable of causing a release of growth hormone that was just as efficient and potent as the reaction from GHRP-6. This reaction was once again seen in swine that were conscious during study, with Ipam releasing growth hormone in the plasma at a rate that is quite similar to GHRP-6. However, in this case there was a higher potency of growth hormone when GHRP-2 was applied, though the efficacy was lower.
• In swine, none of these growth hormone secretagogues affected TSH, FSH, PRL or LH levels in the plasma. Administering GHRP-2 and GHRP-6 increased cortisol and ACTH levels in the plasma but there was no release of ACTH or cortisol levels when Ipam was administered in levels that were 200 times higher than the ED50 for growth hormone release. This research indicates that Ipam would be an ideal candidate for further study for clinical development.

• GHRP-2 and GHRP-6 are not capable of increasing prolactin or cortisol levels but Ipam can selectively release growth hormone, regardless of the size of the application. Mega-applications of Ipam can create a mega-release of growth hormone which can allow the body to release the entire amount of the hormone currently stored in the pituitary gland of the animal. GHRP-6 and GHRP-2 are only capable of allowing an animal’s body to release around 1mcg/kg of growth hormone regardless of how much of the chemical is applied.

• The pharmaceutical industry is particularly interested in any research that assists with targeting or identifying the properties of bioactive peptides in more complex matrices. This can help to assess the feasibility of these peptides in supercritical fluid chromatography of the separation of two pairs of water soluble peptides with the same composition, mass and charge which are only differentiated by the sequence of their amino acids. Evaluating a variety of these conditions, including the conjunction of trifluoroacetic acid, may be the most effective model in noting the elution of isometric pairs of peptides in nitrogenous stationary phases.

• By contrast to this methodology, ammonium acetate and water can create peak shapes in these measurements which are found to be less ideal than additives that are neutral. Basic additives such as iso-propylamine combines with HA-Pyridine can create a high resolution factor that can complete a study on the given peptides. It was found that aminopropyl and HA-pyridine created the best resolution for evaluating these peptide pairs while phenyl-hexyl and silica did not allow for the necessary separation.

• Carbon dioxide and a methanol modifier have been found to assist with peptide solubility transport with stationary phase solvation while HTFA helped to fully protonate the peptide pairs from their ion pair to conjugate the based cationic peptide analate. This separation process was found to be the ideal ion pair supercritical fluid chromatography for this method with Ipam providing the ideal resolution for HA-Pyridine columns for peptides in a neutral state.

Analytial and preparative scales include supercritical fluid chromatography that indicate a widespread applicability for separating chirals of polar pharmaceutical candidates, but this technique is quickly becoming achiral because there is a larger utility for separating ionic analyates, like organic sulfonates and amine salts.

The key to this is including additives like ammonium acetate and trifluoroacetic in the mobile phase of associating with the stationary silica and evaporative light scattering detection. This method was found to create a .3 TFA in methanol and .2 TFA in 90:10 methanols: water and a spike with a 90:10 ratio of Ipam and methanol.

Protein Synthesis, Translation (1):

Protein Synthesis, Translation (2):

Protein Synthesis, Translation (3):

Insulin Growth Factor

Insulin-like growth factor or IGF most commonly refers to somatomedin C or IGF-1 which is a protein that is encoded using the IGF1 gene.

• IGF based peptides contain a large group of chemicals that are used in an animal’s body to communicate physiologic environmental changes or needs. To achieve this, IGF molecules have to cell-surface receptors and ligands which are arranged in a variety of ways, allowing for a six-high affinity of binding proteins and IGFBP degrading enzymes, known as proteases.

• The axis of an IGF molecule is known as the growth hormone/IGF-1axis. IGF-1 is typically secreted by an animal’s liver when it is stimulated by growth hormone and used for regulating the physiology of the body and preventing pathological problems such as cancer. The axis helps to maintain cell proliferation and inhibits cell death when possible.

• IGF-2 is believed to be a primary growth factor that is essential to the early expression of growth hormone while IGF-1 is used to maximize the growth of tissues. Studies on mice have noted these differences and also helped the scientists to realize that IGF-2 is primarily shown in the fetal animals rather than in full grown animals as a means of developing the kidney, brain or liver.

• There are systems which can vary the expression of IGF-1 and growth hormone in the circulation. This may include the genetic makeup of the animal, its sex, age, amount of exercise, nutrition level, stress level, disease state, species, body mass index, xenobiotic intake and estrogen status, among others. IGF-1 is found to regulate the development of neurons— including those for synaptogenesis, neurogenesis, myelination and dendritic branching. It can also be seen assisting with neuroprotection after neurons have been damaged. Children with a higher IQ have been found to have higher levels of serum IGF-1.

• The development of cochlea is also impacted by IGF-1 by managing apoptosis. A deficit of IGF-1 for this system can result in hearing loss. There appears to be a correlation by a lack of IGF-1 and a reduction of hearing and short height in young animals or those entering some form of puberty.

• There are a variety of types of IGF receptors so it can be difficult to narrow down the distinct types of tissues that are affected by IGF-1, particularly when comparing reactions amongst different species of animals. The role of IGF-1 as a neurotrophic factor is perhaps the most well-known because it is easy to tract its induction of survival neurons. In many animals IGF-1 also causes hypertrophy in skeletal muscle because it induces protein synthesis that can block atrophy. In many cases IGF-1 can also protect cartilage by activating osteocytes that can act as an anabolic factor for bone cells. In very high concentrations IGF-1 can act as an insulin receptor and compliment the effects of insulin that is also present in an animal’s system.

• The tissues in an animal’s body will display a variety of IGF-1 receptors based on the effects that would best impact this tissue and induce survival of the neurons that are present in this area of the animal’s body. Common reactions that affect this phenomenon include hypertrophy in skeletal muscle, blocking muscular atrophy protecting cartilage and inducing the synthesis of proteins. IGF-1 is also associated with activating osteocytes which could be anabolic in some animals.

• Both IGF-1 and IGF-2 are regulated by proteins, known as IGF-binding proteins, which are used by animals’ bodies to modulate the IGF receptors that can work both as a means to inhibit IGF by simultaneously promoting the use of IGF in theory by helping the delivery of the chemical to the receptors so that the half-life of IGF-1 is increased. Current data suggests that IGFBPs are essential to the ability of an animal to regulate IGF once it has been activated.

• Recent studies indicate that the IGF/insulin axis is essential to the aging process. Fruit flies and nematodes amongst other animals show an increase in their lifespan when their version of insulin that mimics the gene in mammals is eliminated. It is somewhat difficult to replicate these results in mammals because the production of insulin is linked to a variety of genes and therefore cannot be completely eliminated.

• Simple organisms have fewer IGF-1 receptors and in many cases the roles of these insulin types are unknown. This is largely because these organisms lack specialized organs that would require insulin to react as part of a glucose homeostasis reaction. In addition to this IGF-1 has been found to cause dauer formation to developmental stage C in nematodes which will affect the lifespan of the animal when it is in the larva stage, a situation that lacks any correlation with mammals. Because of this lack of correlation between species it is unclear whether or not IGF-1 will perturb aging in mammals though there is some indication that the phenomena may be related to restrictions in the diets.

• Additional studies are currently ongoing to determine to determine how IGF impacts diseases such as diabetes or cancer. It is currently indicated that IGF may impact the growth of breast or prostate cancer cells though researchers are not currently able to agree how IGF-1 plays a role in this process.

Current difficulties are posed to those that are studying the impact of IGF-1 and IGF-2 and how these things could be utilized in future pharmacological settings because there is such a variance in how these peptides behave in different types of animals.

Today, researchers are not yet ready to begin testing IGF based applications on human test subjects, but the variance of reactions in test subjects such as swine, emus, insects or rats make it difficult to narrow down what types of applications would even be appropriate for human test subjects. In some cases, the use of synthetic human tissues or tissue samples have been helpful in moving around this obstacle until more definitive data can be confirmed.

Structural Integrity of the Blends

Peptides, both short and long, rely on their natural structural integrity to interact with tissues and complete biological tasks, and this is something that a synthetic peptide will need to address in order to have biological significance.

• Data from a variety of animals or human tissue samples indicate that the integrity of myelin sheaths will deteriorate during normal aging, which can result in neural networks that are commonly found throughout the body to become disconnected or otherwise disrupted. This phenomenon can be viewed using an MRI, which is typically used as a way of accessing the impact of things such as Alzheimer’s disease on the brain and other neurological tissues. Evaluating the affected tissues early can help to determine the condition of the genu and the splenium to determine both the presence of the disease as well as the most effective means of addressing these complications.

• Calculating the transverse relaxation rates using an indirect measure of the white matter and the structural integrity of these neurological formations. The relationship between the splenium and genu differed in two main regions of the animal brain. The quadratic function was used to show the accelerating rate of decline in structural integrity starting around 31. The genu was found to deteriorate while the splenium decline was more gradual or liner. This indicates that age related structural damage is heterogeneous and consistent with the myelin properties that are more susceptible to the aging process.

• The function of the brain and signaling throughout the body is also largely reliant on peptides and the release of hormones stimulated from this chemical for normal bodily function in animals. As the aging process begins to impact the structural integrity of animal tissues, the proper function and integrity of the peptides throughout production may also be impacted. Research into these matters will often focus on developing synthetic peptides that could potentially help avert the damage to animal tissue, as a result of such structural damage from the aging process.

• Throughout the past decade there has been a distinct increase in the discovery of bacteria that are resistant to antibiotics, organisms that are generally classified as “superbugs.” These bacteria cannot be killed with current antibiotics that are approved for human use, which poses a threat to public safety, resulting in a pressing need to introduce new, effective medications into the market.

• One potential solution for addressing the growing problem surrounding a lack of credible antibiotics is to generate medications that rely on antimicrobial peptides. These peptides are isolated from living entities which use these peptides to create a defense against bacteria and other organisms that can invade an animal’s body and cause illness. Based on a template of the natural peptide, a variety of antimicrobial cationic peptides can and have been designed to combat pathogens. Each of these peptides can be designed with a diverse structure biologically and chemically, to address specific microbial threats or to be used on different types of organisms for antimicrobial purposes.

• The key to this research is developing new peptide structures that will mimic the natural peptide so they are not rejected by the body, directly address the threat of the incoming microbe and do not pose a potential for dangerous side-effects once they are applied to the animal. Because of these concerns, few peptides have entered clinical animal trials to date, and had success at this stage. The short half-life of many peptides poses a concern when it comes to applying these peptides or storing them in a pharmaceutical setting for medical use later. However, the wide range of biological activities, structures, activity spectra and mode of action those peptides possess shows potential that these issues will be addressed in time.

• Self-assembly and mineralization of peptide nanofibers shows some potential to addressing the issue of creating peptides that are capable of maintaining their structures for an increased length of time. pH induced self-assembly has been applied to peptide amphiphiles to create a scaffold of nanostructured fibers that is similar to an extracellular matrix. This allows the nanofibers within the peptide to be cross-linked in a reversible manner to decrease or enhance structural integrity as necessary.

• After the fibers are cross linked they can be subjected to hydroxyapatite or mineralization directly to create composite minerals that have crystallographic c axes of the hydroapatite are aligned with the fiber’s long axes. This alignment is identical to that which would be seen between hydroxyapatite crystals and collagen in an animal’s bone.

Both long and short peptides are designed to be broken down by the animal’s body as a means of harvesting the chemicals used to create the molecule during biological processes. In some cases, peptides will break down into smaller molecules or peptides that are capable of creating additional processes within the body after their initial task has been completed. Due to this phenomenon, long peptides generally have a more fragile structure than those of the short variety. Understanding where peptides are designed to break and what enzymes or biological interactions can cause damage to the structure of a peptide will help researchers develop synthetic peptides with reinforced or purposely weakened structures that can be used to alter the way the peptide interacts with animal tissues.

Tadalafil

Tadalafil is a PDE5 inhibitor that is researched for its ability to address erectile dysfunction in mammals.

• Originally tadalafil was developed by ICOS but it is now sold worldwide by Lilly ICOS LLC for research purposes. This peptide can commonly be applied to animal test subjects in 40mg applications for best and most accurate results. In some cases tadalafil will be manufactured as Tadacip when it is manufactured in India or provided in smaller applications.

• Tadalafil is often sold under the name Cialis or Adcira for its potential in addressing pulmonary arterial hypertension or benign prostatic hyperplasia. This refers to a combination of erectile dysfunction and benign prostatic hyperplasia that have coincided.

• Penile erection as a result of sexual stimulation is caused by a relaxation of the penile arteries and the corpus cavernosum muscles to allow blood flow to increase to this area. Nitric oxide may mediate this response from the endothelial and nerve terminals to stimulate the synthesis of cGMP in the muscle cells. Smooth muscle can be relaxed by Cyclic GMP to increase the blood flow within the corpus cavernosum.

• Inhibiting phosphodiesterase 5 (PDE5) can help to increase the function of the penis and its ability to achieve an erection by increasing the presence of cGMP, a process that tadalafil is designed to achieve.

• Because sexual stimulation must be present to release nitric oxide, the inhibition of PDE5 from tadalifil will not have any sort of effect on the function of an animal’s penis without sexual stimulation of some kind being present, which is why this peptide is currently administered to animals just prior to sexual activity with an increase in application size as the animal becomes tolerant of the substance.

• This current application schedule would not be effective in a pharmacological setting. Men that would require tadalifil for treatment of erectile dysfunction would need to plan their use of the drug and schedule sexual activities accordingly, which in many cases would not be an effective means in achieving a healthy sex life. To address this problem, clinical trials on animal subjects are currently attempting to determine if it would be possible to create an application of this peptide that could be taken once daily for those that suffers from chronic erectile dysfunction. Some European markets are also attempting to lower the application size of Cialis as a means of allowing humans to use this chemical as part of a therapy for erectile dysfunction, though the product is not yet ready for human use. The potential risks and benefits of these types of applications are currently unknown.

• A variety of trials have been performed on many types of mammal test subjects to determine the side effects of different sized applications of tadalafil. The most commonly seen irritations include muscle aches, back pain, headache, indigestion, flushing of the skin or runny nose. Most of these side effects are conjectured based on the behavior of the given animal. The effects of tadalafil appear to fade after a few hours, though some side effects appear to last as long as 48 hours depending on the size of the application.

• The FDA has discovered that applying tadalafil can lead to vision impairment caused by nonarteritic anterior ischemic optic neuropathy in some test subjects. Most animals that showed signs of this development had underlying vascular or anatomic risk factors that researchers were unaware of when the chemical was first applied. Animals that are older or already show signs of coronary artery disease, diabetes, hyperdension, hyperlipidemia or were exposed to smoke have an additional risk for this side effect. Because so few test subjects showed these signs and were exposed to a variety of factors that could have impacted their vascular health, no conclusions were drawn regarding the dangers of tadalafil on NAION. However, because this trend was noted, any similar PDE5 inhibitors that are already on the market for human use are now required to carry a label that warns of this possible association and doctors must discuss this potential risk with erectile dysfunction patients that hope to receive a prescription to manage their condition.

• In studies tadalafil is commonly compared or introduced with other medications. This can help researchers better determine any risks that may be posed to those using this chemical if it enters the market, particularly if there is success in creating a daily form of tadalafil for chronic conditions. It is currently known that PDE5 inhibitors cause transiently low blood pressure, so it cannot be used along with organic nitrates. Those that are exposed to organic nitrates within 48 hours of an application of tadalafil are at risk for hypotension that could be life threatening. This would be difficult for any humans hoping to use a future daily form of this chemical if they suffered from angina as paramedics would not be able to use many common medications to assist them in the case of an emergency. This issue is one of the concerns that is inhibiting the acceptance of a regular application of tadalafil for humans.

• Predominantly tadalafi is seen to metabolize with hepatic CYP3A4 enzyme systems. The presence of any other chemicals or existing medications that would induce this type of reaction in an animal has been seen to lower the half-life of tadalafil and create a significant reduction in serum levels. This has been seen repeatedly in animal trials.

When compared to similar PDE5 inhibitors including vardenafil and sildenafil, there are a variety of reactions that differentiate tadalafil. Vardenafil and sildenafil are capable of inhibiting PDE6 which is found in the eyes whereas tadalafil is less effective. Sildenafil test subjects are also reported to be more sensitive to light because of this inhibition to PDE6 and have a blue tinge that other test subjects do not.

Vardenafil and sildenafil are also more effective than tadalafil in inhibiting PDE1 which is in the vascular smooth muscle, heart and brain which increases the instances of flushing, vasodilation and tachycardia in test subjects. By contrast, tadalafil is more effective in inhibiting PDE11 than the other PDE5 inhibitors.

Inexpensive Peptide Supplier

Getting the most for your money is essential when investing in peptides for long term research to ensure that there is an adequate supply of chemicals available for large sample sizes and long term trials.

• Much of the cost of manufacturing peptides has little to do with the ingredients necessary to create the compounds as great deal of these peptides are created from very basic materials that are readily available. Much of the cost of preparing a peptide for sale involves researching the potential uses of this peptide in the research market. Most companies that produce peptides are also performing trials of their own to determine their functionality and how they impact the biological processes that animals require to produce and maintain healthy tissues.

• Research into peptides available for sale also depends on creating peptides that can easily be shipped to other research facilities without incident. In animal tissues peptides often have a very short half-life that lasts only a few seconds, just long enough to trigger the necessary biological process, before they are broken down and absorbed by the tissue to take advantage of the nutrients contained in the peptide. In some cases peptides will break down into other molecules that can trigger other biological processes, such as those peptides that trigger muscle growth and hunger, which can impact how a peptide will behave in a research setting. Minimizing this breakdown before the peptide is applied to the animal test subject is essential to getting accurate test results from the product.

• To ensure that there are minimal side effects or breakdown of the chemicals in a research peptide, many companies have altered the structure of a synthetic peptide compared to the originals. This allows them to strengthen the bonds in these peptides to ensure that they will remain intact in the bloodstream of the animal for as long as possible. Some peptides are also altered from their natural forms to attempt to alter how these chemicals will interact with the tissues. Much of the research on the side effects or reactions to these synthesized chemicals are still unknown, so researchers will need to take care to watch subjects closely to ensure they do not provide applications of peptides that are dangerous and alter the effects of their research.

• Another method used to protect the integrity of peptides for shipping is by shipping these chemicals in a freeze-dried state. This prevents any breakdown of the chemical for months or even years while they wait to be used, particularly if researchers keep these containers sealed or frozen. Researchers can then reconstitute small amounts of the peptide as necessary for individual applications to ensure that the chemicals are likely to behave consistently between trials. When ordering chemicals that require reconstituting, read the directions carefully to determine the concentration of the peptide. This will help to ensure that the chemicals are at the proper level and blended with the right liquid modifier to get accurate results without side effects.

• Companies that do not have ongoing trials for peptide research actively on their books are more likely to offer inexpensive peptides than those which are attempting to finish similar research products. Those that are currently performing trials on a given peptide  will need to maintain a higher level of stock to ensure that they have enough to manage their own needs, so will likely sell any additional product they have on hand at a higher price to meet the demand. Comparing prices and maintaining a familiarity with others in the field can help to ensure that a fair price for peptides can be negotiated.

• Companies that produce peptides in mass quantities are also more likely to offer an affordable price. Not all companies are certified or capable of making consistent batches of peptides in large amounts, so it is important to carefully look into which peptide suppliers are the most appropriate for this task. Most who are capable of supplying large doses of peptides will note this in their name or description so that they can readily be identified. Consult with these companies regarding how much of a peptide it is appropriate to purchase at one time so that freshness can be guaranteed throughout the life of a trial.

• Investing in a company that promises quick shipping is also considered essential for those that are hoping to save money on investing in research grade peptides. The less time a peptide spends in transit, the longer it will last during the trial process, allowing scientists to perform additional trials for each round of an experiment without the worry that there will be inconsistency or negative side effects that could affect the level of research performed.

Like any product, it is vital for researchers to carefully compare companies before investing in peptides. To ensure consistency in research, scientists are often advised to invest in peptides from the same suppliers so they contain the same preservatives, require the same solutions for reconstitution and are designed to be constituted at the same level. Seeking out a company that is certified to create large batches of peptides and has years of experience can help to ensure that a research facility can continue to get the research products they require.

Some companies are willing to sign a long term contract with a research facility they know will be performing a research project for a long period of time. This helps to ensure that the company will continue to supply regular shipments with a set payment so that researchers do not have to worry that they will have the tools they need to manage their experiment.

Working out a long term contract helps to ensure consistency of product and shipments that is essential to accurate and reliable research. This provides an opportunity to perform multiple trials or mimic earlier experiments to help ensure that the results achieved are accurate and can be used as a benchmark for future assignments.

 

Resource Box:

http://www.sciencedirect.com/science/article/pii/S019745800300232X

http://www.ingentaconnect.com/content/ben/cdtid/2002/00000002/00000001/art00008

http://www.sciencemag.org/content/294/5547/1684.short

http://www.swolesource.com/forum/evolved-research-supply/1589-ipam-basics.html

http://www.sciencedirect.com/science/article/pii/S0021967311003402

http://www.sciencedirect.com/science/article/pii/S0021967312002671

http://en.wikipedia.org/wiki/Insulin-like_growth_factor_1

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Cytoplasm contains a gel substance called cytosol that is contained within a cell membrane and organelles of animal cells of sub-structures. The contents of any prokaryote organisms that do not have a nucleus will be housed in the cytoplasm, but eukaryote organisms that have a nucleus will separate this material from the cytoplasm in an area known as the nucleoplasm. In most cases the cytoplasm is 70-90 percent water and is a colorless material. Cytoplasm can be jelly like or liquid, though this depends on what type of cell it is in and how that cell is designed to interact with the body.

Most cellular devices occur within the cytoplasm including the metabolic pathways such as cell division or glycolysis. Granular and inner mass known as the endoplasm as well as the clear, glassy outer layer known as the cell cortex and ectoplasm are controlled within the cytoplasm. Movement of calcium ions throughout the cytoplasm are believed to signal metabolic processes and activity in animals. The movement of the cytoplasm around the vacuoles, a process known as cytoplasmic streaming, is used to signal this process in plants.

Constituents of the Cystoplasm

Cytoplasm contains three major elements that are used to signal its effects in different parts of the cells.

• Organelles, or the membrane bound structures within the cells are given specific functions that contribute to the larger functionality of the cell. Major organelles such as the endoplamic reticulum, mitochondria, vacuoles, Golgi apparatus, lysosomes and chloroplasts in plants can be suspended in this area of the cytosol.

• Cortosol is contained in cytoplasm that is found within organelles that are membrane bound. This can make up almost 70 percent of the volume of a cell and contains organic molecules, salts and water. Because the network of fibers and dissolved macromolecules including proteins is very high in cortisol, macromolecular crowding can occur here if the cortosol is not at an ideal solution. This can alter how the components interact.

• Cortosol is made from a combination of dissolved molecules, cytoskeleton filaments and water, though it also contains the protein filaments that create the cytoskeleton and structures including the ribosome, vault complexes and proteasomes. The fluid portion of this type of cytoplasm is known as the endoplasm.

Cytotoplasmic inclusions, small insoluble substances that are suspended within the cytosol are included in a wide variety of cell types in animals as well as silicon dioxide and calcium oxalate in plants which can be converted into glycogen, starch or polyhydroxybutyrate for energy.

These are commonly seen as lipid droplets that are made up of lipids and protein that can be stored as sterols or fatty acids that are used for energy. These lipid droplets are a majority of the adipocyte volume in cells that are specially designed to store lipids, though other cells may hold these as necessary.

Viewing Cystoplasm at a Microscopic Level

Because cells are too small to be seen by the naked eye, there is no way to observe or understand cytoplasm without viewing it through a microscope lens.

• Unlike the organelles of a cell, the cytoplasm does not take on any particular shape or function that can be distinctly recognized, but rather helps to make up the body of the cell which provides additional structure for the vital portions of this body.

• When viewed through an electron microscope the cytoplasm of the cells can be viewed in intimate detail. Animal cells can be seen as a three dimensional shape that contains strands of protein set in a lattice pattern. This is known as the microtrabecular lattice that is designed to interlace with other structures in the cytoplasm to hold other structures in place. This discovery helped scientists to realize that the cytoplasm was not actually a solid mass, but a group of lattes that are interconnected to create something of a fence that would hold together the rest of the cells and prevent the organelles from shifting.

• The cytoplasm contains a cytoskeleton. Not only does this help to provide a shape for the cell but these cytoplasmic filaments help the cell to move as necessary. The alignment of these filaments will vary based on the needs and shape of the given cell.

• The cytoplasm can also contain a variety of salts that are capable of conducting electricity. A cell can use these properties to power the mechanics of the cell as necessary. This function will vary based on how the cell interacts with the rest of the body and the specific task the cell is desired to take on.

In addition to helping to provide structure for the cell, the cytoplasm can be used to dissolve waste and nutrients. The cell can use these filaments to move and move materials throughout the cell by using cytoplasmic streaming, a process that creates a churning motion within the cell body. The nucleus is capable of changing the shape of the cytoplasm, flowing throughout the mass as the cell moves.

Research and Potential Controversies

Cytoplasm and many of the known organelles in a cell are designed from maternal gamete which is still being researched for a full understanding of its functionality.

• There is not a great deal of existing research on the maternal inheritance and cytoplasmic inheritance of mitochondrial DNA compared to genomic DNA or that shared within the cell nucleus.

• Many traits or items within a cell that have been labeled as female have not been researched to their full extent. The cytoplasm is one of the objects that has fallen into this category, with the nucleus being labelled as male and therefore receiving more attention from researchers. This is similar to the sperm and egg being gendered in a full sized animal body. It is generally believed that both the cytoplasm and the nucleus are essential to creating new life through cell division.

Much of the older information surrounding the cytoplasm was based on the idea that it was a passive part of the cell, existing to provide structure for the active elements that encouraged cellular behavior. This outdated understanding of the cytoplasm has been replaced as scientists have discovered that the cytoplasm is used to encourage viscoplastic behavior. This helps to move nutrients in and out of the cell to fuel the other organelles.

This also helps to move the cell as necessary throughout the body so cells may interact with one another. The reciprocal rate of broken bonds within the cytoplasmic network that helps to create these reactions can be measured and reported, creating a better understanding of the different functions of a variety of types of cells within an animal.

Cytoplasm may also be referred to as protoplasm, or the plasma membrane that surrounds the contents of a cell. This is a generalized term for the water, amino acids, ions, nucleic acids, lipids, proteins and other items that make up this substance. Cytoplasm is more often used to describe the protoplasm in eukaryotes. It is believed that eukaryotes contain a protoplasm that is divisible into a cytoplasm that provides structure and is controlled by the nucleus of a cell that has developed electron microscopy.

This was originally believed to be because cytoplasm was a homogeneous fluid that simply existed to help cells maintain their shape, but it is now known that cells contain a variety of substances and organelles that assist with the functionality of the cell.

 

Resource Box:

http://en.wikipedia.org/wiki/Cytoplasm

http://www.fi.edu/qa97/biology/cells/cell3.html

http://en.wikipedia.org/wiki/Protoplasm

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Chemicals within the body pose an interesting challenge to researchers that have been tackled in a variety of ways throughout the history of biochemicals. Chemicals within the body contain the same elements and atomic properties that they would in any other setting. However, within organic tissues chemicals take on a unique roll that is not seen anywhere else in nature. These chemicals not only help to compose natural tissue, they are produced by natural tissue to provide a variety of functions that are essential to the function of the body as a whole. What continues to prove to be a challenge for scientists is the fact that chemicals do not behave in an identical manner within different animals, in spite of containing the same molecular structure— and in many cases interacting with tissue with the same makeup. There have been a variety of methods used to help better predict molecular behavior so organic chemicals may be better defined. This process starts with creating a failsafe method for identifying and organizing chemicals discovered in organic tissue so they can be monitored and better understood— based on their properties and their behavior. This can also help to structure experiments that will create artificial versions of these chemicals to be introduced to organic tissue, as a means of controlling biological behavior.

Amino Acid Molecular Weight

Amino acids are commonly identified by their molecular weight, as a means of categorizing unknown chemicals along with chemicals that scientists work with frequently.

• Amino acids are the second largest component of cells for muscle and other tissues, only being surpassed by water. These amino acids can play a variety of roles within the body, but they are most often seen acting in biosynthesis functions or working as neurotransmitters.

• An amino acid is defined as an organic acid that is formed from carboxylic acid and amine. These are a functional group of chemicals, each with a side chain that can be formed from nitrogen, hydrogen, oxygen or carbon among other elements that can be used as an identifier for the chemical; each of these side chains is unique to the amino acid. These side chains also help to identify the role of the amino acid in an animal as it interacts with organic tissue.

• To date, there are around 500 known amino acids which are widely classified by weight, but they are also often classified in to gamma, delta, beta or alpha groups— based on their polarity level. Side chains of amino acids are grouped— based on the additional elements that are included in the chain, such as sulphur or hydroxyl. These groups are commonly listed as aromatic, aliphatic or acyclic.

All molecular weights are noted in g/mol

• Alanine- 89.1

• Arginine 174.2

• Asparagine- 132.1

• Aspartate- 133.1

• Cysteine- 121.2

• Glutamate- 147.1

• Glutamine- 146.2

• Glycine- 75.1

• Histidine- 155.2

• Isoleucine- 131.2

• Lysine- 146.2

• Methionine- 149.2

• Phenylanine- 165.2

• Proline- 115.1

• Serine- 105.1

• Threonine- 119.1

• Tryptophan- 204.2

• Tyrosine- 181.2

• Valine- 117.1

Each molecular weight, also referred to as molecular mass, is the specific mass of a given molecule. This is calculated by adding the sum of the mass for each atom known to be contained in the atom multiplied by the number of atoms in the molecule. Being able to define the molecular mass will help to determine the size of a molecule, which are then classified as a small, medium or large grouping. Small or medium molecules are defined using spectrometry which will help to determine the stoichiometry. This is how amino acids would be defined. Large molecules, such as full proteins, can be defined using a variety of method– such as light-scattering or viscosity.

• In most cases, it is more appropriate to refer to a molecule’s relative molecular mass because molecules are often given an assumed mass— based on their relevancy to C. Molecular and atomic masses are dimensionless but are measured in Daltons which can help to define the number for a mass of one molecule, when divided by the mass of C. This will help to provide a molar mass for chemicals so scientists are able to work with a unit gram of the substance.

There are 20 amino acids that have been defined as those that play a vital role in the function of an animal’s body. Of these 20, nine have been determined to be essential to the body because they cannot be created by compounds within the body itself, and therefore must be taken in from other substances. In many cases this helps to regulate the diet of an animal because they must consume foods that contain the proper balance of chemicals to create the necessary amino acid compounds. The specific amino acids that are essential to an animal will vary between species; therefore there is a wide variety of dietary needs. These may also change as the animal ages, presenting a more interesting look at the differences in the functions of animals. Understanding the classifications of these peptides will help to better understand which chemicals are being utilized by animals and how they are built or broken down— based on the interactions they have with the natural tissues.

Short Peptide

Peptides are groups of amino acids that work to make up different proteins within the body, each of which take on a unique roll when interacting with bodily tissues.

• Peptides are formed by short amino acid monomers that are linked by different types of peptide bonds to create a chemical chain. When the carboxyl groups of different amino acids interact with each other, it can create a variety of covalent chemical bonds that will join different amino acids together. The number of amino acids joined in this pairing will help to define what type of peptide has been formed: a dipeptide, tripeptide, tetrapeptide, etc.

• It is important to note that peptides differ from proteins— based on how they function in the body as well as their size. As a general rule a peptide must contain 50 or fewer amino acids in order to receive this classification, whereas proteins will often contain significantly more, or may even be made up of multiple peptides that break off and act independently as the molecule interacts with bodily tissues or moves through the bloodstream. Proteins will also often bind to ligands to create cofactors or coenzymes which can create completely different proteins or grow to create macromolecules that take on a variety of roles within an animal.

• Scientists are currently working on learning more about the techniques that peptides use that differ from how proteins interact with the body. It appears as though there are size boundaries that help to determine whether or not a polypeptide or a protein may take on a certain role within the body. The specifics of this are still being learned— as many peptides react differently within different groups of tissues or even within different animals that have similar characteristics. It is also not uncommon to see peptides react differently within male or female animals within the same kingdom.

• If an amino acid becomes incorporated into a peptide it is referred to as a residue because this type of reaction will typically result in the release of hydroxyl— from the existing carboxyl in the amino acid. This will lead to the formation of water molecules every time an amide bond is made. All peptides have this type of reaction, with the exceptions of cyclic peptides. These, instead, create a residue known as C-terminal or the N-terminal—depending on the type of peptide that the chemicals are reacting with and the type of process that is being generated.

• There are no specific rules that distinguish proteins from polypeptides. In general, a long peptide that contains amyloid beta data can be classified as a protein, but small peptides, particularly those in the insulin family, are rarely reclassified into the protein group.

• Within the peptide group, peptides are divided into long chain peptides or short chain peptides. Long chain peptides, or ogliopeptides, may be split into shorter peptides as they are used in a variety of processes throughout the body. These are commonly seen within natural substances, creating the various biological processes that are necessary for the functionality of this tissue. When absorbed or created in animal tissue, they will begin to create a variety of new processes that can assist with tissue engineering. The animal’s body will use the various elements used in the chemical bonds of a peptide to repair, enhance or replace biological tissue or functionality as necessary.

• Short peptides have a similar functionality to long chain peptides, but they are significantly smaller in size. They are sequentially bonded but do not contain any proteins. There is no specific number of amino acids that will allow a scientist to classify a chemical as a short or long chain peptide, though in general those with only two amino acids are placed in the short category.

• Both short and long chain peptides secrete hormones which can allow an organism to trigger different biological processes within their body. These hormones can also be used to produce different biological processes as necessary. Short chain peptides tend to be more stable than long chain peptides because there are fewer bonds included in their construction, so in many cases these peptides are considered the ideal system for encouraging a biological process that would require longer or higher levels of stimulation to complete. Long chain peptides, instead, are designed to be broken down and absorbed by the affected tissue as the given biological process continues.

• Because short chain peptides are more stable, they have a longer shelf life, which makes them an ideal source for biological research. Short peptides are also more efficient when they enter an animal’s body, so it is much easier for scientists to track the changes that these chemicals are making in the body. For this purpose, short chain peptides are commonly the ones produced synthetically, as a means of testing the functions or the reactions of these chemicals on the tissues. While long chain peptides are occasionally used for this research, their length often causes them to collapse shortly after being applied to the tissue, which can result in inconsistent results or data during experimentation.

• Short chain peptide research generally focuses on the idea of being able to create biocompatible materials that will help to reduce the effects of the aging process on animal tissue. Amino acids that are used to create peptides can be harnessed by the body and used to complete biological processes more efficiently. If the body is not producing or being exposed to the necessary amino acids to maintain the tissues, scientists hypothesize that applications of peptides containing the proper amounts of the necessary peptides could be applied as a means of ensuring that the body continued to function at the right level, or even an enhanced level if desired.

• Short peptides are considered ideal for this potential biological application. Short peptides are often referred to as self-assembling peptides which contain the active motifs that the animal tissue would need to create a variety of processes. The short peptides that are capable of bonding without stimulation are able to replicate information that was created in the animal tissues and independently create or transmit this information as necessary. This allows the peptide to take on a variety of natural processes that do not require them to change their structure or alter their behavior within the body, which can include the stimulation of tissue engineering when the body is going through a period of growth.

• The idea of generating new tissue by stimulating the body using peptides is only in its initial stages. Both short and long chain peptides are being used to determine the potential effectiveness of this research, but as of today, most numbers that are associated with this practice are considered to be preliminary data that will be used to fuel pharmacological research in the future.

In an even more interesting biological system, it has been shown that peptides may, in some cases, mimic the reactions or processes of proteins in the body, helping the organism perform processes at a higher level of functionality— in spite of the normal regulations that their body may have. Scientists hope to harness this ability and use peptides in pharmacological applications in the near future, as a means of correcting diseases or deficiencies in humans. Research on animal test subjects often revolves around the application of synthetic peptides for this purpose, though this research will continue to be enhanced in upcoming years as scientists continue to understand the role different peptides play in animal tissue and how this reaction might vary if the peptide is synthetic in nature.

Polypeptide Chain

Peptides are divided into a variety of classes— based on the chains that are used to create them and the processes that they take on once they are created or enter an animal’s body.

• Ribosomal peptides are some of the most commonly synthesized peptides, though they are naturally synthesized from mRNA translations in an animal. These peptides will often reach their mature form by entering proteolysis, which higher organisms will use as a way of signaling molecules with hormones to generate the necessary biological processes the body requires. In some cases organisms can use these amino acids to create natural antibiotics including mocrocins, with the residues of these amino acids being used by the ribosome as necessary to create the biological process involved.

•  More commonly, ribosomal peptides will enter into posttransitional modifications including glycosylation, palmitylation, sulfonation, phosphorylation or hydroxylation. These are generally a linear process, though some structures have created a lariat bonding structure during a specific set of biological processes. Some animals are able to use these amino acids to create a more exotic reaction– such as converting D-amino acids from L-amino acids to create venom in platypuses. These unique variations of the use of chemicals generate the need for far reaching research into the use of peptides, as every animal may react to the application of a synthetic peptide in a slightly different manner.

• Nonribosomal peptides are created by enzymes that are unique to that particular peptide instead of by the reactions of a specific set of ribosomes within the body. Glutathione is the most common peptide in this group, which is used by animal tissue to create an antioxidant defense that can be used to help protect the tissues from aerobic organisms. Additional common nonribosomal peptides include fungi, plant or unicellular organism processes that can be used to synthesize modular enzyme complexes which are known as nonribosomal peptide synthetases.

• Nonribosomal peptide synthetases usually contain a similar structure, but they will take on a variety of different modules to deal with chemical manipulations within the different types of tissue where they have been placed. This also helps a variety of organic tissues creating the necessary bonds to manufacture this product, given the natural properties the tissues possess. Nonribosomal peptides are typically cyclic and have complex structures, but there have been discoveries of linear nonribosomal peptides as well. The bonding of these peptides is commonly compared to the mechanics that are necessary to create polyketides or fatty acids in more complex organisms. It is not uncommon to see the bonding of a hybrid compound of these structures for this very reason. Compounds that were synthesized in this manner include thiazoles or oxazoles.

• Milk peptides are created with proteins from milk and are broken down using enzymes in the body, typically the enzymes that are used to fuel digestion, to create lactobacilli. This is the bacteria that are responsible for the fermentation process in milk.

• Peptones are those that are derived from meat or milk products that are digested using proteolysis. These contain small or short chain peptides that can be broken down or absorbed to create spray dried material such as salts, fat, metal, vitamins and a variety of biological compounds. These peptides are considered the essential building blocks that fungi or bacteria will be used to grow within a nutrient media.

• Peptides are starting to become a prominent field of study within molecular biology for a variety of reasons. The peptides may be used to create peptide antibodies within an animal without needing to purify the protein that is to be used. This can be performed very simply by synthesizing antigenic peptides of the protein or sections of protein the scientist would like to work with. This can then be used to create antibodies against the given protein. Such research is commonly performed on mice or rabbits.

• Peptides are also becoming a subject of interest for scientists because they are instrumental in the research of mass spectrometry that allows researchers to readily identify proteins based on their sequence or their mass, greatly speeding up the time initial research takes to discover the necessary structures for a biological process. Peptides that are created the most frequently for this purpose include those in-gel digestions which are created after the electrophoretic separation of the given proteins.

• Research surrounding peptides are often used as a means of studying the function and structure of a variety of proteins. Synthetic peptides are often used as probes to discover where the bonds or interactions between proteins and peptides occur. The bonding of peptides must be replicated exactly, in order to ensure that these biological processes will react the same way they would in a natural setting, which has fueled additional research into the structure of the given peptides, particularly for those processes that require long peptides to complete. In some cases, scientists are shortening or enhancing the bonds on synthetic peptide structure in order to achieve the stability necessary to create a biological process that can easily be monitored or replicated in the tissues.

• Research into inhibitory peptides may eventually be used in clinical research to help inhibit cancer proteins or those from other diseases. There is a promising application of peptides that may be able to target and inhibit LHRH. In this case the peptides will act as an antagonist that will interrupt the way the LHRH cells can bind to the receptors on natural tissues within the body. Inhibiting these structures could be helpful in managing conditions such as prostate cancer, if research in animal test subjects continues to progress in a promising manner. At this time additional investigations will be necessary in order to determine if peptides truly have attributes that could be used to fight cancer. The properties that have currently been observed by peptides or similar structures are not yet at a point where it can be considered definitive.

As peptides are used by the body, they will be broken down which creates a variety of fragments. Peptides fragments can be used to identify the different proteins that were the source of this chemical reaction and quantify this protein source to help scientists better understand how to trigger these biological processes. In many cases, the products that are commonly used during enzymatic degradation would break down and make use of these peptides that can be replicated on controlled samples in a laboratory setting, but it can be difficult to compare these reactions to paleontogical or forensic samples, because other natural effects can impact or degrade these samples before the process can be accurately observed.

Where to Buy Peptides

In order to continue research into the understanding and potential use of peptides and their components in medical research, scientists will require a steady stream of peptides to work with during their experiments.

• In order to gain access to natural peptides, scientists will need to be able to control their release in the animal’s body and then harvest the chemicals as a means of further detecting their impact on tissues or other biological processes. This is not particularly practical, as peptides are naturally released in small amounts that are only designed to trigger a specific biological response. Furthermore, most peptides have a very short half-life, causing them to begin to break down almost immediately after being released to the body.
• To counteract this problem, a variety of companies have begun to create synthetic versions of highly known and used peptides. These peptides are bonded in the same chemical fashion as the original peptide to ensure accuracy when applied during medical research. However, additional research must continue, as some synthetic peptides have been found to react differently than the natural counterpart they have been modeled to replicate. This is largely theorized to be the result of external application of the artificial peptides, which are independent of the chemical and hormonal process that would trigger the use of this peptide in a natural setting.

• Peptide manufacturing companies will often sell large quantities of these peptides as a means of providing researchers with the samples they require to perform long-term research studies. Acquiring peptides from a singular source is considered ideal or even essential for many researchers because this allows them to guarantee a consistency of product that is necessary for research grade chemical tools. This also helps to guarantee that the bonding and chemicals used to produce the synthetic peptide will match, better ensuring a consistent performance as it is applied to animal test subjects.

• Much of the concern regarding using artificial peptides in research is ensuring that the product remains stable for as long as possible. This will help to ensure a consistent performance with the lowest possible level of side effects in animal test subjects. Some peptides have been altered as a means of controlling their half-life, to minimize their breakdown during transport or storage. Peptides are also freeze-dried and reconstituted as necessary as a means of ensuring that a premature breakdown will not occur.

• Any company that is not shipping peptides in a frozen, temperature controlled state runs the risk that their product will become damaged during transit: thus they may not be suitable for research-grade use. Similarly, these products should contain clear instructions for how they should be reconstituted, when they should be used and how they should be stored to ensure a maximum level of efficiency from the product.

• While a significant amount of care must be put into the transit and storage of these chemicals, peptides are not in fact difficult or costly to produce. Many who overcharge for peptides are simply attempting to take advantage of the popularity of these products in current research fields.

• Similarly, some companies add binders or preservatives to peptides as a means of lengthening the time that they can be shipped. However, these additional ingredients can interfere with the results noted when the chemicals are applied to animal tissues.

Maxim sells peptides at a reasonable price so it is easy for researchers to get the high amounts of product they require— in order to complete their research fields. These peptides are stored, sealed and shipped in a way that guarantees to preserve the chemical composition of the product. Peptides are also shipped very quickly as a means of ensuring that there will not be a breakdown in the chemical structure before the researcher has been given adequate opportunity to use these products. No additional ingredients are added to any samples during the production processes to ensure accurate research results.

CJC 1293

CJC 1293 is often referred to in conjunction with the similar peptide, CJC 1294 which is commonly synthesized as a means of controlling biological research throughout the bodies of animal test subjects.

• CJC 1295 is a tetrasubstituted peptide hormone that consists of a 30 amino acid chain. This peptide will generally function as an analog to growth hormone releasing hormone GHRH. ConjuChem of Canada created this hormone as a means of better synthesizing this process. CJC 1295 was created as a means of improving the function of rHGH or GHRH research because CJC 1295 is able to bioconjugate with substances, such as serum albumen, which will increase the half-life of the product as well as the potential therapeutic window. This is accomplished by protecting groups of amino acids that would normally be susceptible to degradation from enzymes within GHRH.

• By contrast, CJC 1293 is the natural growth hormone releasing hormone GRF or GHRF which is also commonly classified as somatocrinin or somatroliberin. This is a hormone that is designed to trigger the release of growth hormone. This peptide contains 44 amino acids and is produced naturally within the arcuate nucleus of the hypothalamus in an animal. The first indications of GHRH are commonly seen in the hypothalamus between 18-29 weeks of gestation which is used by the body to produce growth hormone and somatotropes as necessary within the fetus.

• CJC 1293 is released from neurosecretory terminals in nerves which are within the acruate neurons. This peptide is then carried through the hypothalamo-hypopyseal portal system that will deliver the chemicals to the anterior pituitary gland to stimulate the secretion of growth hormone from the growth hormone releasing hormone receptor. CJC 1293 is released in a pulsatile manner which will help to stimulate a similar release of growth hormone.

• CJC 1293 is also used by animal tissue to promote slow wave sleep in a way that is more direct than some other biological methods. Growth hormone is required for any postnatal growth or any growth of bone tissues. It is also used to help regulate carbohydrates, proteins and the metabolism.

• If CJC 1293 is bound with GHRHR. It can increase the production of growth hormone, but this is largely due to the fact that the cAMP dependent pathways will be increased as well as the phospholipase C pathways within minor pathway structures.

• cAMP dependent pathways are initiated by CJC 1293 binding to it receptors which will cause a confrontation with the receptors that activate G alpha subunits within G-protein complexes on the intracellular side. In turn this will cause a simulation of the adenylyl cyclase as well as an increase in the intracellular cyclic adenosine monophosphates that allows any free subunits to translocate to the nucleus of the different phosphorylate and transcription factors of the cAMP response element binding proteins.

• Together with phosphorlylated CREB and its coactivators CREB binding protein and p300 there is an enhancement of the transcription of growth hormone that allows for the binding or CREB proteins and cAMP response elements within the promoter region of growth hormone genes. This will also increase the transcription of GHRHR genes that can provide positive feedback on this process.

• Within phospholipase C pathways CJC 1293 stimulates the phospholipase C throughout the By complex heterotrimic G-proteins. The activation of PLC will continue to produce inositol triphosphate and diacylgylcerol which will in turn lead to the release of intracellular Ca that stems from the endoplasmic reticulum. This will increase the concentration of Ca that will cause vesticle which will release the secretory vesticles that contain a premade growth hormone serum. In some cases the influx of Ca will have a direct impact on the cAMP release that will create a distinct reaction that varies from the traditional cAMP dependent pathways that are used by animal tissue to activate protein kinase A.

• The activation of GHRHR using CJC 1293 is also used as a way to open Na channels by deploying phosphatidylionositol 4 and biphosphate 5. This will cause cell depolarization and this change can cause the intracellular voltage to open and trigger a voltage dependent calcium channel. When this process is completed it can result in a vesticle fusion that will in turn release growth hormone to the necessary stimulated tissues.

• Additionally, CJC 1293 can be expressed in a way that is demonstrated within peripheral tissues and cells that surround the main site of the hypothalamus. This type of reaction has been seen in gastrointestinal tracts of animals and the epithelial mucosa. In some pathological studies a similar reaction has been seen in the cells of tumors.

 

• CJC 1293 is a lead compound in a variety of functional and structural analogs including that of CJC 1295. There are a variety of analogs that are used in research chemicals for specific applications that require CJC 1293 to maintain their structure. Semorelin is one of the most common functional fragments of CJC 1293 which is designed to assist with the diagnosis of deficiencies of growth hormone secretions. Tesamorelin is another analog that is designed to help diagnose lipodystrophy in those suffering from HIV with a highly active antiretroviral therapy program. As of 2011, research is focusing on the effects that these chemicals could have on elderly animals before it can be widely used for experimentation or use in humans.

CJC 1293 and the reactions it has within animal tissue are opposed by somatostatin (also referred to as growth hormone inhibiting hormone). When somatostain is released from its neurosecretory nerve terminals which are designed to act as a perinventricular somatostain neuron; this chemical will be carried through the hypthalao-hypophysical portal circulation. This will deliver somatostain to the interior pituitary where it will cease the effectiveness of CJC 1293 and inhibit the secretion of further growth hormone. If CJC 1293 and somatostatin are released alternatively it can increase the pulsatile secretion of growth hormone. A great deal of research is dedicated to comparing these reactions as a means of understanding how to control or replicate  this processes using synthetic versions of these chemicals. With research into peptides and the potential to create synthetic versions of of these materials still very much in its infancy there is a great deal of potential for creating new therapeutic measures that will help to manage a variety of conditions or diseases that damage animal tissues. As researchers grow to understand the functionality and composition of peptides that much better, it is all the more likely that they will be able to create chemical structures that mimic or enhance the abilities of these chemicals to react with animal tissues. Research on tissue samples or animal test subjects currently indicates that there is a promising pharmacological component to these processes that could see use in humans in the upcoming years if all research continues to progress as planned.

Resource Box:

http://www.lifetechnologies.com/us/en/home/references/ambion-tech-support/

http://en.wikipedia.org/wiki/Amino_acid

http://en.wikipedia.org/wiki/Molecular_mass

http://en.wikipedia.org/wiki/Peptide

http://www.maximpep.com/research/differences-between-long-and-short-peptide-chains/

http://en.wikipedia.org/wiki/CJC-1295 http://en.wikipedia.org/wiki/CJC-1293

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The peptide blend CJC-1295/Ipamorelin is a combination of the peptides CJC-1295 and Ipamorelin.  The two peptides are combined in a single container, and are available for purchase solely for scientific study and research based on animal test subjects.

The Breakdown of Each Peptide

Simply stated, CJC-1295 and Ipamorelin can be blended into one dual combination because neither peptide blocks the functionality of the other.  What’s more, while their methodology is different, scientific study on animal test subjects has determined that each peptide in essence causes the same physiological function to occur.  Namely, both peptides have been shown to facilitate an elevated rate of secretions related to muscular and skeletal tissue growth in animal test subjects. CJC-1295 performs this increase by its ability to boost the peptide’s bioavailability; that is, the rate in which it is absorbed.  Additionally, it also works to extend the half-life of the peptide, from less than seven minutes to more than seven days.  It has the ability to perform the latter function due to a scientific process that is known as bioconjugation.  This relatively new technology is defined by its facility to take a reactive group and bond it to a peptide, causing a reaction that eventually leads to a more stable bond.  This stable bond in turn enables an extended prohibition of natural degradation, thus causing an extension.  In the case of CJC-1295, this extension of the half-life is especially significant.  These processes allow for an increase in the secretions that are tied to growth, which in turn lead to a more efficient building of muscular and skeletal tissue amongst animal test subjects. Ipamorelin, on the other hand, functions in a way that is rather similar to the peptide GHRP-6; a peptide that scientific study on animal test subjects has also proved to be quite effective in blending with CJC-1295.  Ipamorelin chiefly stimulates the secretions that cause growth from the pituitary gland; the pea-sized gland that essentially acts as the heart of the endocrine system.  As it stimulates these secretions, it also blocks the production of the peptide responsible for halting the expression of these very same secretions, thus allowing a higher rate of secretions to occur.  Additionally, Ipamorelin also works in conjunction with the liver to increase growth secretions by stimulating the production of the growth-related peptide IGF-1. The primary function that separates Ipamorelin from GHRP-6 is something that Ipamorelin doesn’t do.  That is; Ipamorelin does not work to stimulate the production of ghrelin; the stomach peptide responsible for causing the sensation of hunger in animal test subjects.  What this means is, animal test subjects do not display an increased desire to consume food after Ipamorelin has been expressed.

Blended Benefits

A key reason why the blending of CJC-1295 and Ipamorelin is being studied scientifically on animal test subjects is due to the numerous theoretical benefits that the peptides have exhibited when blended.  Some of these benefits seem obvious based on both peptides’ functionality, such as an increase in muscular and skeletal tissue, quicker injury recovery, and the strengthening and rejuvenation of joints.  However, other benefits may not be as obvious, such as an improvement of deep sleep and improved skin tone.  Additionally, the residual increase of the secretions related to growth gives animal test subjects the added benefit of increased protein synthesis, which in turn enables the test subjects to break down body fat on a more efficient basis. Minimal Side Effects Unlike other blends that feature CJC-1295, the blend of CJC-1295 and Ipamorelin has exhibited a minimal amount of side effects, per scientific study conducted on animal test subjects.  This is because scientific research on animal test subjects built around CJC-1295 has derived very minimal side effects.  These side effects include:

• Fatigue
• Light-headedness
• Retention of Water
• Headaches
• Temporary numbness of extremities

What’s more, these side effects have been determined to be mild in nature. Still Set Aside for Scientific Research As further study is conducted on the blended peptides CJC-1295 and Ipamorelin, it should be stressed that all research relating to the combined peptide has been solely derived from scientific study and research based on animal test subjects.  Any and all findings that are related to this blended peptides should be considered data that has been culled from its current standing as being in the research phase.  As a result, and study or research involving the peptide blend of CJC-1295 and Ipamorelin should exclusively be contained to the restrictive confines of a strictly regulated environment, such as a medical research facility or a laboratory.

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Ketotifen fumarate is classified as an antihistamine peptide.  It is sometimes referred to as Zatidor, Apo-Ketotifen, Novo-Ketotefin, or Alaway.  It is a sterile ophthalmic solution that presents itself as a finely crystalline powder.  It has a molecular weight of 425.50.  The peptide is also readily soluble in water.

Mechanics of Ketotifen Fumarate

According to scientific study that has been conducted on animal test subjects, Ketotifen fumarate contains properties that are related to antihistamines.  This means that the peptide has the ability to inhibit the action of histamines, which are organic nitrogen compounds that trigger inflammatory responses as a means to ward off various foreign pathogens.  Ketotifen Fumarate also acts as a mast cell stabilizer, which means that it has the capability to control or prevent specific allergic disorders.

In addition to its anti-histaminic abilities, Ketotifen Fumarate acts as a functional leukotriene antagonist.  What this means, is that the peptide has the ability to inhibit the fatty compounds produced by the immune system which in turn constricts airways and causes inflammation in asthma and bronchitis.  Ketotifen Fumarate acts as a functional phophodiesterase inhibitor.  What this means, is that the peptide has the ability to prohibit one or more of the five subtypes of enzyme phosphodiesterase (that is, PDE), which in turn prevents the inactivation of the intracellular second messengers cyclic adenosine monophosphate (that is, cAMP) and cyclic guanosine nonophosphate (that is, cGMP) by the respective subtypes of PDE.

Theoretical Benefits of Ketotifen Fumarate

Scientific research that has been conducted on animal test subjects in relation to Ketotifen Fumarate has determined that the peptide could provide several theoretical benefits.

Some of these theoretical benefits associated with Ketotifen Fumarate include:

• Prevention and treatment of allergic conjunctivitis.  Because of Ketotifen Fumarate’s ability to promote anti-histaminic activity coupled with its capability to block or inhibit certain allergic responses, scientific study on animal test subjects have concluded that the peptide can be effective in blocking the onset of allergic conjunctivitis.  This condition is marked by the inflammation of the conjunctiva, which is the membrane covering the white part of the eye.  Other symptoms of the condition include the production of tears, itching, redness due to vasodilation, and swelling of the conjunctiva.  These types of reactions are associated with a host of various triggers, such as pollen, grass, dander, ragweed, and animal hair.
• Prevention of asthma attacks.  Because Ketotifen Fumarate has the ability to act as a leukotriene antagonist, it can theoretically work to prohibit the fatty compounds that produce constricted airways and inflammation from manifesting within an animal test subject.  This in turn would enable for the prevention of the inflammation that causes asthma and bronchitis.
• Alleviation of the symptoms related to irritable bowel syndrome.  Some of the symptoms that Ketotifien Fumarate can work to alleviate in relation to irritable bowel syndrome include chronic abdominal pain, bloating, discomfort, and alteration of bowel habits.

Potential Negative Side Effects of Ketotifen Fumarate

While scientific research that has been conducted on animal test subjects have determined several theoretical benefits in relation to Ketotifen Fumarate, there have also been a few potential negative side effects that have been associated with the peptide.

Some of more serious potential negative side effects relating to Ketotifen Fumarate include:

• Excessive redness of eyes
• Excessive drainage of eyes
• Swelling of eyelids
• Swelling of tongue
• Swelling of throat
• Difficulty breathing
• Severe dizziness
• Eye infections
• Eye pain
• Changes in vision
• Severe itching in the eyes

Some of the less serious potential negative side effects relating to Ketotifen Fumarate include:

• Mild burning in eyes
• Mild stinging in eyes
• Eye irritation
• Dryness of the eyes
• Increased sensitivity to light
• Drowsiness
• Weight gain
• Dry mouth
• Increased irritability
• Increased instances of nosebleeds

Other scientific studies based on laboratory rat subjects indicated that the administration of the peptide reduced the rate of copulation and fertility index amongst the subjects.

For Scientific Use Only

While there have been  several scientific studies that has been conducted on animal test subjects in order to determine Ketotifen Fumarate’s range of operational mechanics, theoretical benefits, and negative side effects, it should be noted that any and all findings that are associated with the peptide are still considered to be the product of current laboratory research.  Because Ketotifen Fumarate is currently in the research phase, any study or usage relating to the mechanics, operations, benefits, and side effects of the peptide should solely be contained to the restrictions of a strictly controlled environment such as a medical research facility or a laboratory.

 

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GnRH, also known as Gonadotrophin-releasing hormone, is a trophic peptide hormone responsible for the discharge of follicle-stimulating hormone (also known as FSH) and luteinizing hormone (also known as LH) from the anterior pituitary.  Because of its connection to luteinizing hormone, it is sometimes known as Luteinizing-hormone-releasing hormone (LHRH) or luliberin.

Functionality of GnRH

GnRH is produced within the hypothalamus, which is the portion of the brain primarily responsible for the linking an animal test subject’s nervous system with its endocrine system through the pituitary gland.  Its primary function is to regulate the secretion of hormones that are linked to the development, growth, pubertal maturation, and reproductive processes found within an animal test subject’s body.  It is produced by neurons and secreted in a pulsatile fashion into the cardiovascular system.  The frequency and the amplitude of these pulses determine the secretions of follicle stimulating hormone and luteinizing hormone from the pituitary gland, with lower frequencies triggering the former secretion and higher frequencies triggering the latter secretion.

Scientific research on animal test subjects has determined that GnRH assists in inhibiting the rapid degradation of the hormones that are associated with the peptide.  These studies also yielded greater and longer surges of the hormone’s release amongst the subjects during the follicular stage.  The surges were also marked with a larger pulse frequency.  This, in turn, enables for a more regulated and predictable secretion of hormones related to the presence of GnRH.

GnRH and Biological Research

Scientific study based on animal test subjects relating to the use of GnRH has focused on theories in how its use may someday be instrumental in the use of several conditions.  Some of these conditions include:

• The treatment of hormonally sensitive cancers:  Scientific study based on animal test subjects has determined that GnRH may theoretically play a key role in the controlling or staving off various cancers that are associated with hormonal sensitivity.  These types of cancers include breast cancer and prostate cancer.
• The treatment for precocious puberty:  Scientific study based on animal test subjects has led to the theory that GnRH’s regulatory properties in relating to the secretion of hormones can cause a delay in the onset of puberty amongst subjects who may experience this particular kind of growth spurt too soon.
• Improved management of female disorders dependent on the production of estrogen:  Scientific study based on animal test subjects has determined that GnRH and its regulatory properties can play a substantial role in managing conditions that are caused because of irregularities relating to the production of estrogen.  These conditions include endometriosis, uterine fibroids, adenomyosis, and menorrhagia.
• Treatment of severe cases of congenital adrenal hyperplasia:  This type of treatment can relate to several autosomal recessive diseases that result from the mutation of genes for enzymes mediating the biochemical steps of production of cortisol from cholesterol by the andrenal glands.

Scientific research conducted on animal test subjects has also determined that the introduction to GnRH has led to the temporary suppression of fertility in male dogs.  It has also led to the induction of ovulation in mares.

The Side Effects of GnRH

Scientific research based on animal test subjects has yielded several theories relating to potential negative side effects that may be connected to the peptide.  These theoretical negative side effects include:

• Hypoestrogenism – Because of the link that GnRH has with the secretion of FSH and LH hormones, it is theorized through research on animal test subjects that a lapse in its regulatory properties may lead to issues related to a lack of estrogen in the system.  These conditions may include headaches, hot flashes, and osteoporosis.  It should be noted that in these cases, it is theorized that estrogen could be given back via an “add-back regimen” to combat the adverse effects.
• Increased risk of cardio trauma – Scientific research conducted on animal test subject has led to the theory that the usage of GnRH in conjunction with fighting specific types of hormonally sensitive cancers could increase the risk of heart problems by as much as 30%.

For Scientific Use Only

While there have been plenty of information generated in relation to GnRH’s operations, mechanics, theoretical benefits, and theoretical negative side effects, it should be noted that any and all research and findings related to the peptide is still solely derived from scientific study based on animal test subjects.  Because it is a still in the research phase, any research or usage done with the peptide should solely be contained to the restrictions of a strictly controlled environment such as a medical research facility or a laboratory.

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Hexarelin is a peptide that can promote the secretion of certain hormones.  It is a hexipeptide that consists of six amino acids that can release certain hormones as they are needed.  It has a half-life of about 70 minutes.

Hexarelin’s Mechanics

Hexarelin’s mechanics behind its functionality is currently unknown.  However, it has been determined that the peptide acts on two parts of the brain region in animal test subjects:

• The pituitary gland – The pea-sized gland that regulates the endocrine system.
• The hypothalamus – The part of the brain that acts as a link between the endocrine system and the nervous system.

Despite the unknown nature of Hexarelin’s mechanics, scientific studies have been conducted on animal test subjects to determine its effects.  These studies have derived several different effects linked to its use, including elevated levels of fat loss, connective tissues, density of bone minerals, meiosis, mitosis, and elasticity.  In turn, these effects have led to animal test subjects experiencing improved endurance, joint rehabilitation, wound healing, and improved muscle strength.  Studies also conclude that Hexarelin’s functionality can last a long stretch of time.

Furthermore, scientific studies on animal test subjects have determined that Hexarelin does not induce an increased desire for food consumption.  The peptide achieves this because it does not increase the levels of ghrelin; the amino acid peptide that clears out the gastric system and induces hunger.

Further scientific studies have also determined that the peptide promotes an increase in the secretion of IGF-1 from the liver of animal test subjects.  This additional secretion plays a key role in breaking down fat and improving strength.

While it is known that Hexarelin’s functionality is associated with acting upon specific parts of the brain, its effects on the brain have yet to be determined.

The Heart and Hexarelin

Scientific study on animal test subjects has determined that there seems to be a link between the peptide and the heart.  This research suggests that the peptide could include specific properties that cause certain effects that protect the heart.

Specifically, these finding were based on a study using lab rats with certain hormone deficiency in comparison to other lab rates.  The purpose of the study was to determine if an administered dose of Hexarelin could lower the risk of cardiac dysfunction; a condition that had been determined to be a side-effect of select certain hormone deficiencies.

As part of the study, several lab rates had their pituitary glands removed and given Hexarelin for a week.  Throughout the study, the injections were determined to prevent an increase of left ventricular pressure in the heart.  Furthermore, it was determined to lessen reactivity of the coronary vasculature to angiotensin II and coronary perfusion pressure.  It was also determined that the peptide could inhibit the release levels of prostacyclin while the heart contracted.

These conclusions indicated a strong instance of the peptide being able to lessen the damage that can occur to the cardiovascular system when it occurs as part of a certain hormone deficiency.  While this conclusion is currently rooted in clinical research, additional study may eventually produce a use for Hexarelin in relation for preventative care for growth-hormone issues relating to cardiovascular-based issues, such as recovery after a heart attack.

Other Determinations Concerning Hexarelin

Although scientific study on animal test subjects has derived several positive outcomes, there have been a few negative side effects which have been linked to the peptide’s use.  This includes water retention, bone pain, muscle pain, and elevated diabetes risk.

Additionally, other studies built around animal test subjects have been conducted in relation to Hexarelin’s functionality.  For example, some studies have been conducted to study the potential protective effect the peptide may have on the central nervous system after an injury.  The basis of this study is a direct link to how Hexarelin acts on the hypothalamus.  Other studies built around Hexarelin’s functionality include:

• Caspase 3 – This particular protein has been determined to be a vital and essential component in normal brain development in addition to its typical function in apoptosis.
•  Akt – Also referred to as protein kinase B, this protein controls cellular survival and regulates metabolism.
• Extracellular signal-regulated kinases – These particular protein molecules are essential tools in the regulation of meiosis, mitosis, and postmitotic functions within differentiated cells.

It should be noted that all studies and findings that are related to Hexarelin have been due to scientific research conducted on animal test subjects.  Any research relating to Hexarelin and its functionality should be contained to the restrictions of a strictly controlled environment such as a laboratory or a clinical research facility.