TB500 is a synthetic chemical that is based on the similar peptide which is developed in a vast majority of animal cells, known as thymosin beta 4. The synthetic version of this chemical is designed to take on the most beneficial properties of theymosin beta 4– including collagen deposition, endothelial cell differentiation, keratinocyte migration, angiogenesis in the dermal tissue and decreasing inflammation in an animal’s body.
One of the key mechanisms of the synthetic TB500 is that it has been found to be beneficial in regulating cell building proteins, specifically actin which is an essential component in the development of circulating cell tissue throughout the body. TB500 is capable of binding with actin and can also block actin polymerization. This is an actin-sequestering molecule within the eukaryotic cells in an animal.
Development and Key Factors
TB500 was first identified as a gene, but was later up-regulated to a four to six fold during the formation of blood vessels as it was found that it could promote growth in a majority of circulating cells and tissues within the body.
- This peptide is classified as a ubiquitous family of 16 molecules with similar properties which show high conversion sequences and localization in the circulating cells.
- TB500 can be found in a great deal of wound fluid and if a synthetic version of the chemical is applied to an animal subcutaneously it can promote wound healing, but building of muscle tissue and decreasing the amount of time that it takes for cells within muscle fibers to recover.
- A key feature of TB500 is noting that it promotes cell migration by creating a unique interaction with actin, throughout the cell cytoskeleton.
- The characteristics of TB500 were discovered by increasing migration of keratinocytes and the endothelial cells. This will also increase an animal’s production of extracellular matrix-degrading enzymes.
Research has confirmed that there is a potency to TB500 which can be used to naturally promote wound repair and also contains anti-inflammatory properties. This works differently than other known repair factors including growth factors because TB500 promotes keratinocyte and endothelial migration.
This peptide also fails to bind with the extra cellular matrix and contains a low molecular weight which allows this peptide to travel longer distance when it is within tissues.
Because TB500 is a synthesized version of the naturally occurring thymosin, there are a few different methodologies for creating this peptide for research settings.
- Most of the artificial versions of this chemical are designed to be used in experiments that will investigate its role in regulating actin. This means that many synthetic versions of these chemicals are designed to be used in applications that will maximize this potential.
- There are thousands of similar proteins that naturally occur in animal cells. If you will be performing experiments that are focusing on the potential uses of TB500 it is important to invest with a laboratory that synthesizes TB500 without diluting it with other chemicals that has similar properties.
- TB500 naturally makes up approximately10 percent of the cells that it occurs in naturally. Given this high amount of peptide that will naturally be in an animal’s body it will be necessary to account for this when making purchases of supplies that will be applied to live animal test subjects.
Peptide companies provide careful analysis for peptides to ensure that you select a facility that will provide research grade product. Most laboratories which synthesize these chemicals do so using methods that will ensure purity and consistency amongst the samples.
Selecting Proper Sources
There are many similar product on the market which opens the potential for those that are hoping to compare the properties of TB500 to similar peptides.
- The genetic makeup of the synthetic version of TB500 that is used for research into the development of actin in animals is patented, so buying from a facility that uses this specific branding and ensures that this is a synthesized version of thymosin beta 4 and not a closely related natural peptide will help to guarantee accurate results.
- Any live animal test subjects will need to be treated with care when they are exposed to TB500. This is intended to migrate through a variety of tissues which offers much more variety in the application of the peptide, but this also must be kept consistent in order to not hinder results. You may also find that it is necessary to alter application patterns, based on how the peptide affects tissues in between the targeted zone.
Research has noted the potential anti-inflammatory properties of TB500 while encouraging growth factors. This may react to several tissues even in a close radius which could generate unpredictable, though not necessarily dangerous, results.